Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
نویسندگان
چکیده
منابع مشابه
Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
Several microscopy techniques are available today that can detect a specific protein within the cell. During the last decade live cell imaging using fluorochromes like Green Fluorescent Protein (GFP) directly attached to the protein of interest has become increasingly popular. Using GFP and similar fluorochromes the subcellular localisations and movements of proteins can be detected in a fluore...
متن کاملLive Cell Imaging in Fission Yeast.
Live cell imaging complements the array of biochemical and molecular genetic approaches to provide a comprehensive insight into functional dependencies and molecular interactions in fission yeast. Fluorescent proteins and vital dyes reveal dynamic changes in the spatial distribution of organelles and the proteome and how each alters in response to changes in environmental and genetic compositio...
متن کاملAutomated quantitative live cell fluorescence microscopy.
Advances in microscopy automation and image analysis have given biologists the tools to attempt large scale systems-level experiments on biological systems using microscope image readout. Fluorescence microscopy has become a standard tool for assaying gene function in RNAi knockdown screens and protein localization studies in eukaryotic systems. Similar high throughput studies can be attempted ...
متن کاملLive-cell Imaging Using Fluorescence Microscopy
The ever increasing abundance of different fluorescent proteins and vital dyes that are available to researchers today make it possible to study a large variety of dynamic biological processes in live-cells [1, 2]. Many proteins of interest can be studied by expressing fluorescent fusion proteins in cells, either transiently by transfection or viral infection or permanently using transgenic ani...
متن کاملNegative regulation of meiotic gene expression by the nuclear poly(a)-binding protein in fission yeast.
Meiosis is a cellular differentiation process in which hundreds of genes are temporally induced. Because the expression of meiotic genes during mitosis is detrimental to proliferation, meiotic genes must be negatively regulated in the mitotic cell cycle. Yet, little is known about mechanisms used by mitotic cells to repress meiosis-specific genes. Here we show that the poly(A)-binding protein P...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2019
ISSN: 1940-087X
DOI: 10.3791/59822